purified human catd (Enzo Biochem)
90
Structured Review
Enzo Biochem
purified human catd
Purified Human Catd, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/purified+human+catd/purified+human+catd/pmc10996108-62-7-11
Average 90 stars, based on 1 article reviews
Purified Human Catd, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/purified+human+catd/purified+human+catd/pmc10996108-62-7-11
Average 90 stars, based on 1 article reviews
purified human catd - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis"
Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis
Journal: Alzheimer's Research & Therapy
doi: 10.1186/s13195-024-01443-6
Figure Legend Snippet: Inhibition of CatD slows the catabolism of tau in vitro and in cultured cells. ( A ) Representative Coomassie blue-stained polyacrylamide gel loaded with recombinant human tau (rTau) incubated for the indicated times with recombinant human CatD (5 nM) in the absence or presence of equal concentrations (1 µM) of Aβ40 or Aβ42. ( B ) Quantification of rTau levels as a function of time in 4 independent experiments. Note how rTau catabolism is unaffected by Aβ40 but markedly slowed by Aβ42, a potent competitive inhibitor of CatD. Data are mean ± SEM; n = 4. ( C ) Overview of the experimental approach used to quantify hTau catabolism in “Tet-Off” cultured neuroblastoma cells (see main text). ( D ) Representative western blot showing hTau levels (stained with antibody P44) at different time points after cessation of hTau expression in the absence or presence of the CatD inhibitor, pepstatin A (PepA; 1 µM). ( E ) Quantitation of hTau levels as a function of time from 6 independent experiments. Note the marked increase in the half-life of hTau in the presence of PepA (0.98 days; 95% CI 0.80 to 1.25) relative to DMSO-treated controls (0.51 days; 95% CI 0.429 to 0.627; P = 0.0012). Data are mean ± SEM, n = 6
Techniques Used: Inhibition, In Vitro, Cell Culture, Staining, Recombinant, Incubation, Western Blot, Expressing, Quantitation Assay
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Purification:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Activity Assay:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Incubation:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Inhibition:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified In Vitro:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Cell Culture:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Staining:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Recombinant:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Western Blot:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Expressing:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified Quantitation Assay:Article Title: Cathepsin D regulates cerebral Aβ42/40 ratios via differential degradation of Aβ42 and Aβ40 Article Snippet: Aβ peptides and PepA were diluted in neutral Dilution Buffer (20 mM Tris, pH 8.0 supplemented with 0.1% BSA), with addition of DMSO as appropriate, and reactions were initiated by transfer into Assay Buffer (60 mM Na-citrate; 80 mM Na 2 HPO 4 , pH 4.0; Sigma) supplemented with purified Article Title: Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis Article Snippet: Reactions were initiated by addition of purified |